Bromelain is a group of thiol‑hydrolytic proteases extracted and purified from the pineapple plant, Ananas comosus, using biotechnological methods. It has a molecular weight of 33,000 Da and an isoelectric point of 9.55. Its molecular structure contains an oligosaccharide composed of xylose, fucose, mannose, and N‑acetylglucosamine. Bromelain is primarily derived from the plant’s stem, which is why it is also referred to as stem bromelain. The main active component of bromelain is a thiol‑containing protease; it also includes peroxidase, acid phosphatase, several protein inhibitors, and bioactive calcium. Its catalytic site is a thiol group (—SH), enabling it to hydrolyze various proteins and participate in biochemical reactions. Consequently, bromelain finds broad applications in food processing, pharmaceuticals, health foods, animal husbandry, and other biotechnological industries.

Product Features

• Bromelain is derived from natural plants and is characterized by its safety, non-toxicity, and broad spectrum of activity.
• Utilizing advanced bioengineering technologies, the product is manufactured through microfiltration, ultrafiltration, membrane separation, and vacuum freeze-drying, meeting both food-grade and pharmaceutical-grade standards and having obtained national certification for compliance with hygiene standards for food export enterprises.

Product Characteristics

Pale yellow or white solid powder

Terms of Use

• Bromelain exhibits a relatively low optimal reaction temperature, ranging from 37 to 60°C, with its most favorable temperature being 50 to 60°C.
• The optimal pH range is 6.0–8.0.

Application areas

• Bromelain is a natural, non‑toxic protein with no adverse side effects. As a thiol‑dependent protease, it catalyzes the hydrolysis of proteins, cleaving peptide bonds to yield polypeptides or amino acids and producing a protein hydrolysate with a distinctive flavor.
• Bromelain has been shown to be a safe and effective therapeutic agent. In the medical field, it can inhibit platelet aggregation, promote fibrinolysis, exert anti-inflammatory and antitumor effects, modulate cytokines and immune responses, facilitate skin debridement, enhance the absorption of other drugs, dissolve mucus, aid digestion, accelerate wound healing, and improve cardiovascular and circulatory function. Bromelain is well absorbed when administered orally, and evidence suggests that its therapeutic efficacy is enhanced at higher doses.

Vitality

Definition of enzyme activity: Ultraviolet spectrophotometry: Under the assay conditions (37 ± 0.2°C; pH 7.0), the enzyme activity is defined as the amount of enzyme required to hydrolyze casein at a rate that produces trichloroacetic acid‑soluble material with an absorbance at 275 nm equivalent to that of 1 µg of tyrosine per minute, expressed in units per gram (u/g).

Product Standard

This product complies with the food-grade enzyme specifications set forth in the United States Pharmacopeia (FCC) and the Chinese Pharmacopoeia.

Product Specifications

• Refined Bromelain 500,000 U/g
• Refined Bromelain 800,000 U/g
• Refined Bromelain 1.2 million U/g

(Enzyme activity units of GDU/g or CDU/mg can also be provided upon customer request.)

Storage

• It is recommended to store in a cool, dry place away from light, at temperatures below 0°C.
• Prolonged storage or unfavorable storage conditions can lead to a reduction in enzyme activity to varying degrees; if temperature and humidity are too high, the dosage should be appropriately increased during use.

Safety

• Bromelain is a purely natural enzyme preparation; it is a protein. Consuming foods containing enzyme preparations is akin to eating protein-rich foods—beneficial and harmless to the human body.
• For certain sensitive individuals, direct ingestion of highly concentrated enzyme powder or exposure to aerosolized droplets may trigger allergic reactions, and prolonged contact may irritate the skin, eyes, and mucous membranes. During handling, it is recommended to wear protective equipment such as masks and eye shields. Any leftover or spilled enzyme powder should be promptly disposed of; for large spills, gently sweep the powder back into its container, while small amounts can be vacuumed up or cleaned by wetting with water.
• Bromelain is a bioactive substance that is readily affected by heavy metal ions (Fe ³⁺ , Cu ²⁺ , Hg ⁺ , Pb ⁺ The inhibitory and destructive effects of such substances and oxidizing agents should be avoided during storage or use.

Packaging

Aluminum-plastic pouch packaging, 1 kg × 10 pouches per carton; 1 kg × 20 pouches per carton.